Isolation and Characterization of Mesenchymal Stem Cells Derived from Duck Embryo Liver
A number of studies have shown that mesenchymal stem cells (MSCs) are multi-potential cells that can proliferate and differentiate into other cell types. MSCs transplantation has wide prospect in clinical application. Here we summarized the biological characteristics of MSCs from duck embryo liver. MSCs were isolated from duck embryo liver (LMSCs) with collagenase type IV digestion method. A series of assays about biological characterization of MSCs including the identification and differentiation potential were conducted. Primary LMSCs were sub-cultured to passage. MSCsrelated gene CD44, CD105, CD166, IL-7 were detected out. The induced cells, osteoblast, lipoblast, hepatocyte and neural cells performed typical staining and expressed specific genes. This research has indicated that the MSCs from duck liver could be a novel alternative source for experimental and clinical applications.
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Document Type: Research Article
Publication date: August 1, 2017
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- Journal of Biomaterials and Tissue Engineering (JBT) is an international peer-reviewed journal that covers all aspects of biomaterials, tissue engineering and regenerative medicine. The journal focuses on the broad spectrum of research topics including all types of biomaterials, their properties, bioimplants and medical devices, biofilms, bioimaging, BioMEMS/NEMS, biosensors, fibers, tissue scaffolds, tissue engineering and modeling, artificial organs, tissue interfaces, interactions between biomaterials, blood, cells, tissues, and organs, regenerative medicine and clinical performance.
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