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Repairing Effect of a Nano-Artificial Bone Combined with HIF-1α Transfected Bone Marrow Mesenchymal Stem Cells for Rabbit Radial Bone Defects

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Objective: Bone marrow mesenchymal stem cells (BMSCs), as seed cells, were infected with lentiviruses carrying hypoxia-inducible factor-1α (HIF-1α and combined with nano-hydroxyapatite (Nano-HA) artificial bone. The composite artificial bone was implanted to the defect site of rabbit radius to investigate the repairing effect of HIF-1α on bone defects. Methods: Recombinant HIF-1α lentiviral plasmids were constructed and transfected into 293Ta cells. Rabbit tibial bone marrow was aspirated to isolate and cultivate BMSCs using adherent culture method. The cultivated cells were identified by morphological observations and flow cytometry. BMSCs were infected with lentiviruses harboring HIF-1α, and co-cultured with Nano-HA to obtain HIF-1α-eGFP/BMSCs/Nano-HA artificial bone. The artificial bone cultured in vitro was implanted to the bone defect site of rabbit radius. Thirty New Zealand white rabbits were used and randomly and equally divided into 3 groups. At week 12 after an operation, the rabbit radius specimens were determined by gross observations, X-rays imaging, and pathological tests to analyze the healing status of the radial bone defects. Results: 1. At 48 h after the transfection of lentiviral plasmids harboring HIF-1α into 293Ta cells. At week 12 after the operation, the specimens of various groups were detected by gross observations, X-ray imaging, and histological method. Results showed that both HIF-1α-eGFP/BMSCs/Nano-HA composite artificial bone in group A and BMSCs/Nano-HA composite artificial bone in group B could promote the bone defect repair. Furthermore, the HIF-1α-eGFP/BMSCs/Nano-HA composite artificial bone in group A formed more new bones, and therefore had a stronger bone-defect repairing ability than group B. In group C, osseous union was not present in the bone defect site, indicating the failure of bone defect repair. Conclusion: BMSCs function as the seed cells of bone tissue engineering to facilitate osteogenesis. HIF-1α genes promote the BMSCs to stimulate osteogenesis and angiogenesis, and increase the formation of new bone tissues, thus leading to more effective bone defect repair. With the good osteoconductivity of Nano-HA, HIF-1α-eGFP/BMSCs/Nano-HA composite artificial bones possess.
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Document Type: Research Article

Publication date: 01 October 2016

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  • Journal of Biomaterials and Tissue Engineering (JBT) is an international peer-reviewed journal that covers all aspects of biomaterials, tissue engineering and regenerative medicine. The journal focuses on the broad spectrum of research topics including all types of biomaterials, their properties, bioimplants and medical devices, biofilms, bioimaging, BioMEMS/NEMS, biosensors, fibers, tissue scaffolds, tissue engineering and modeling, artificial organs, tissue interfaces, interactions between biomaterials, blood, cells, tissues, and organs, regenerative medicine and clinical performance.
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