Skip to main content
padlock icon - secure page this page is secure

Single-Dosed Genotoxicity Study of Gold Nanorod Core/Silver Shell Nanostructures by Pig-a, Micronucleus, and Comet Assays

Buy Article:

$110.00 + tax (Refund Policy)

As an increasing number of nanoproducts enter our daily life, their potential health risks have caused widespread concerns and have also promoted studies of nanotoxicity. Among these investigations, the genotoxicity of nanomaterials has attracted more attention. Engineered silver nanorod with gold core and silver shell ([email protected] NR) was used in this study to investigate the possible genotoxicity and genotoxicity patterns in peripheral lymphocytes and hepatocytes introduced by released Ag+ and the nanoparticle itself by integrating the Pig-a gene, micronucleus, and comet assays. Most of the [email protected] NR was rapidly cleared from the circulatory system of Sprague Dawley rats, and a small amount of [email protected] NR was retained in the liver for at least 14 days. Our data confirmed that clastogenicity was the primary genotoxicity type induced by [email protected] NR, and both NR particles containing Ag and the released Ag+ contribute to genotoxicity. [email protected] NR was shown to be a clastogen through the introduction of increased %Tail DNA in peripheral lymphocytes (5.82%±0.25%) and hepatocytes (4.83%±0.17%) and promotion of the formation of micronuclei in hepatocytes (1.12%±0.13%) 3 days and 14 days after dosing, respectively (P < 0.05), which could be a result of both Ag+ and Ag shell of the [email protected] NR. However, [email protected] NR was not shown to be a mutagen, as the average RBCCD59– count was not changed significantly as compared with control. These data suggest the importance of adopting appropriate genotoxicity testing strategies in identifying the genotoxicity of nanoparticles in vivo.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: COMET ASSAY; GOLD NANOROD; IN VIVO GENOTOXICITY; MICRONUCLEUS; PIG-A; SILVER NANOPARTICLE

Document Type: Research Article

Publication date: November 1, 2018

More about this publication?
  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more