Nanocochleates as the Potential Delivery Systems for Oral Antitumor of Hydroxycamptothecin
It remains a major challenge to improve the oral antitumor efficacy of hydroxycamptothecin (HCPT). The aim of this study was to develop the oral HCPT formulation via nanocochleates technology. After HCPT was incorporated into calcium ions-induced nanocochleates, its oral bioavailability significantly enhanced, up to a 2.3-fold. Most importantly, in vivo antitumor effect of HCPT-loaded nanocochleates could be achieved upon oral administration to hepatoma-bearing BALB/c-nude mice, showing the remarkable inhibition of tumor growth as compared to normal saline, HCPT suspensions and HCPT-loaded liposomes. Caco-2 cells, as an in vitro model, were conducted to investigate the absorption mechanisms of intestinal tract. Caco-2 transport studies indicated that HCPT-loaded nanocochleates were uptake via clathrin- and caveolin-mediated endocytosis. Furthermore, ZO-1, F-actin and claudin-4, known as three cellular tight junction proteins, were obvious down-regulation, which suggested that HCPT-loaded nanocochleates could open cellular tight junctions and paracellular route. In conclusion, nanocochleates served as a promising vehicle to facilitate the oral delivery of HCPT.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
Document Type: Research Article
Publication date: July 1, 2018
More about this publication?
- Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Terms & Conditions
- Ingenta Connect is not responsible for the content or availability of external websites