Intraocular Fate of Polycaprolactone Nanoparticles Administered via Intravitreal and Various Periocular Routes: Bioimaging of Integral Nanoparticles Using Environment-Sensitive Fluorophores
Intraocular delivery of sustained-release polymeric nanoparticles provides an option for the treatment of intraocular diseases afflicting the posterior segment. However, the development of these delivery systems is hampered by the lack of understanding of their intraocular fate. In this study, polycaprolactone nanoparticles with particle size of 80, 200 and 800 nm were administered via either intravitreal or periocular routes. Tracking of the nanoparticles was achieved by labeling with environment-responsive near-infrared fluorescent probes that are able to identify integral particles from the bulk probe signals, whereas a conventional non-environment-responsive probe cumarin-6 (C6) was simultaneously loaded to mimic the cargoes. Intravitreal injection results in higher levels of intraocular nanoparticles than both retrobulbar and sub-tenon administration. Nanoparticles are able to migrate to retina against little resistance upon intravitreal injection, but either the nanoparticles or C6 are eliminated from retina very quickly. On the contrary, only a limited amount of nanoparticles can permeate across sclera and reach retina via the periocular routes. The periocular sites serve as depots for sustained release of the cargoes, which however gradually permeate into the retina through the trans-scleral pathway and are retained there for prolonged duration. Since the overall retinal retention of the cargoes is similar for either intravitreal or periocular administration, it is concluded that the periocular route is more preferable than the intravitreal route because of the safety issues.
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Document Type: Research Article
Publication date: August 1, 2017
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