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Induction of Innate and Adaptive Immunity with Polyion Complex Nanoparticle Adjuvant Carrying Human Immunodeficiency Virus Type-1 gp120

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The development of safe and effective vaccines against human immunodeficiency virus type 1 (HIV-1) infection is mandatory to suppress the global AIDS pandemic. We have recently created polyion complex (PIC) nanoparticles (NPs) using the anionic polymer poly(γ-glutamic acid) (γ-PGA) and the cationic protein protamine. Both materials are biodegradable. HIV-1 gp120-carrying PIC NPs (gp120-PIC NPs) were prepared by mixing γ-PGA-graft-L-phenylalanine ethylester polymer with protamine containing gp120 antigen in phosphate-buffered saline, and their effect on the induction of innate and adaptive immune responses was examined in mice. gp120-PIC NPs were efficiently taken up into dendritic cells (DCs). PIC NPs upregulated surface costimulatory molecule expression and cytokine production through the mitogen-activated protein kinase-mediated nuclear factor κB signaling pathway in DCs. The immunization of mice with gp120-PIC NPs resulted in robust induction of antigen-specific CD8+ T cells as well as IgG antibodies. In contrast, such induction was not observed, when mice were immunized with gp120 alone or gp120 plus Alum-base adjuvant or incomplete Freund's adjuvant. Furthermore, PIC NPs could induce antigen-specific effector and central memory CD8+ T cells. These results suggest that PIC NPs have potential as an effective vaccine adjuvant against various infectious diseases including HIV-1/AIDS.
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Keywords: ADAPTIVE IMMUNITY; ADJUVANT; DENDRITIC CELLS; HIV-1; INNATE IMMUNITY; POLYION COMPLEX NANOPARTICLES; VACCINE

Document Type: Research Article

Publication date: July 1, 2017

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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