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The Morphology of Self-Assembled Lipid-Based Nanoparticles Affects Their Uptake by Cancer Cells

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The morphology of nanoparticles (NPs) has been presumed to play an important role in cellular uptake and in vivo stability. This report experimentally demonstrates such dependence by using two types of uniform-sized self-assembled lipid-based NPs, namely nanodiscs and nanovesicles, composed of identical lipid composition. The morphology is characterized by small angle neutron scattering, dynamic light scattering and transmission electron microscopy. Both NPs have similar bio-stability in serum and cellular cytotoxicity. However, cellular uptake of the nanodiscs at 37 °C is consistently and significantly higher than that of the vesicles according to the uptake results of several human cancer cell lines, i.e., CCRFCEM, KB, and OVCAR-8, indicating a strong morphological dependence of cellular internalization. Further studies on such morphological dependence using CCRF-CEM reveals that vesicles only use Clathrin- and caveolae-mediated endocytic pathways, while nanodiscs also take the additional routes of macropinocytosis and microtubule-mediated endocytosis.
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Keywords: BICELLES; CANCER CELLULAR INTERNALIZATION; MORPHOLOGY-TARGETING; SANS; SPONTANEOUS FORMATION; VESICLES

Document Type: Research Article

Publication date: October 1, 2016

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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