Production of Nanoscale Vibration for Stimulation of Human Mesenchymal Stem Cells
Mechanical stimulation is becoming a common technique for manipulating cell behaviour in bioengineering with applications in tissue engineering and possibly regenerative therapy. Living organisms show biological responses in vivo and in vitro to various types of mechanical
stimulation including vibration. The development of apparatus to produce vertical motions of nanoscale amplitude is detailed and their effect on mouse endothelial (Le2) and human mesenchymal stem cells (hMSCs) is investigated. Piezo ceramic actuators and aluminium reinforcement were utilised
along with laser interferometry to ensure amplitude consistency at the nanometre level across a cell culture substrate. Peak force applied to the cells was estimated to be of nN magnitude at frequencies of 500 and 1000 Hz. Morphological changes in the cytoskeleton were found for both cell
types along with increased MSC proliferation after 1 week of stimulation at 500 Hz. Changes in the nuclear size of MSCs after stimulation were also found.
Keywords: ACCELERATIVE FORCE; MECHANOTRANSDUCTION; MESENCHYMAL STEM CELLS; NANOSCALE VIBRATION; TISSUE ENGINEERING
Document Type: Research Article
Publication date: 01 July 2016
- Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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