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One-Step Assay for Optical Prostate Specific Antigen Detection Using Magnetically Engineered Responsive Thin Film

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We report a novel and very simple method for the detection of proteolytically-active prostate specific antigen (PSA) at a very low concentration using a self-assembled monolayer (SAM) of magnetic beads conjugated with peptide. The application of surface plasmon resonance (SPR) allows real-time optical characterization of the constructed recognition monolayer. This set-up is also capable of rapid monitoring of PSA samples through an instantaneous detection of medium refractive index shift induced by the enzymatic cleavage of the peptide and the dissociation of free magnetic beads. The results showed the ability of our SPR sensor to detect PSA within a short time (few minutes to 30 min) with high sensitivity (100 pg/ml) and specificity. Thanks to the elimination of washing and blocking steps, as well as the signal amplification and labeling procedures, this approach can be implemented in other miniaturized configurations such as screen printed electrode and biochip array amenable to low-cost point-of-care devices.
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Keywords: BIOSENSOR; MAGNETIC BEAD; PROSTATE SPECIFIC ANTIGEN; SELF-ASSEMBLED MONOLAYER; SURFACE PLASMON RESONANCE

Document Type: Research Article

Publication date: June 1, 2014

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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