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Simultaneous Detection of SERS and Fluorescence Using a Single Excitation for Microbead-Based Analysis

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We demonstrate simultaneous detection of surface-enhanced Raman scattering (SERS) and fluorescence signals from a silver microbead. For the dual signal generation, silver microbeads with a diameter of 15 μm were functionalized with benzenethiol (BT) as a Raman tag and a cardiac troponin I (cTnI) antibody on their surface. SERS and fluorescence signals were obtained using a single argon laser source with 488 nm wavelength. The SERS signals from Raman tag can be used as identification indices for decoding a particular microbead, while the fluorescence signals provide the information about molecular interactions with a specific biomarker. With simultaneous detection of SERS and fluorescence signals using single excitation on the functionalized microbeads, we successfully showed the possibility of a simple barcoding strategy for multiplex analysis using suspension arrays.
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Keywords: FLUORESCENCE; MULTIPLEX ANALYSIS; SILVER MICROBEADS; SIMULTANEOUS DETECTION; SURFACE-ENHANCED RAMAN SPECTROSCOPY (SERS)

Document Type: Research Article

Publication date: July 1, 2013

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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