Micropatterning of Mammalian Cells on Indium Tin Oxide Substrates Using Ion Implantation

In this study, a simple surface patterning method to create micropatterns of mammalian cells on indium tin oxide (ITO) substrates was developed using ion implantation. Thin polystyrene (PS) films spin-coated on an ITO glass was selectively implanted with accelerated proton ions through a pattern mask and then developed to generate PS micropatterns. Wellorganized negative PS patterns were generated on the ITO glass. The results of the in vitro cell culture on the PSpatterned ITO glass with two types of cancer cell lines revealed the formation of well-defined cell patterns through a selective cell adhesion and proliferation only onto the ITO regions separated by PS regions. This facile method for cell patterning may be used to create a desired platform for cellular device applications, such as biosensors and cell microarrays.