Skip to main content
padlock icon - secure page this page is secure

Fabrication of Microengineered Templates and Their Applications into Micropatterned Cell Culture

Buy Article:

$106.67 + tax (Refund Policy)

We present a fabrication method of PDMS microtemplates and their applications into a localized culture of primary mammalian cells. Three types of microtemplates of microwell, microstencil and microcontact printing stamp (mCP) were fabricated, and their feasibilities into cell culture were studied. All of microtemplates were fabricated by PDMS casting from SU-8 mold prepared by photolithography. The pattern used in the test was 500 μm dots. In the culture test of microstencil, a stencil was placed on a glass disk coated with Poly-D-Lysine (PDL) layer. In the other test of microcontact printing, PDL layer was patterned by microcontact printing. The mammalian cells of mouse embryonic fibroblasts (MEF) and cortical neuron were successfully cultured into micropatterns by using the microtemplates. The results showed that cells could be cultured into micropatterns with precisely controlled manner at any shapes and specific size for bioscience study and bioengineering applications.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Publication date: March 1, 2013

More about this publication?
  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more