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Colorimetric Assay of Melamine Based on the Aggregation of Gold Nanoparticles

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Under certain acidic condition, thioglycolic-acid-modified gold nanoparticles (Au-NPs) can react with melamine, resulting in the aggregation of Au-NPs through electrostatic interactions between –COO groups on the surface of Au-NPs and −NH3 + groups of melamine. The aggregation extent depends on the concentration of melamine, which can be validated by SEM imaging and UV-vis spectra. During the aggregation procedure, color change can be observed. Based on the color change, a simple, fast, cheap and visual colorimetric method of detecting melamine is developed, which can be effectively applied to detect melamine in milk powder and develop a new diagnostic kit of melamine.
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Keywords: AGGREGATE; ELECTROSTATIC INTERACTION; GOLD NANOPARTICLES; MELAMINE

Document Type: Research Article

Publication date: October 1, 2011

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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