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Superior Wound Healing Effect of Topically Delivered Silver Nanoparticle Formulation Using Eco-Friendly Potato Plant Pathogenic Fungus: Synthesis and Characterization

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Biosynthesis of silver Nanoparticles (NPs) using microorganisms has been reported but methodologies of synthesis are slow and the silver nanoparticles are not stable. In this study, an attempt has been made to synthesize the silver nanoparticles (SNPs) within 10 min from Phytophthora infestans and it was found to be stable. The SNPs formation was confirmed by changes in colour and also from the UV spectral analysis. The sizes of the particles were confirmed by Transmission Electron Microscopy (TEM), particle size measurement. The Fourier transform infrared spectroscopy confirmed the presence of protein as the stabilizing agent surrounding the SNPs. The physical stability was also tested, and the stability of the nanoparticles was confirmed by zeta potential measurement and by SDS-PAGE profiling. Once the SNP ointment was prepared; the wound healing activity of the SNPs was studied by measuring wound contraction ability in excision wound model at different time intervals and histopathology, the least effective dose of SNP 0.125% (w/w) ointments showed significant wound healing as compared to standard silver sulphadiazine ointment currently available in the market.
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Document Type: Research Article

Publication date: October 1, 2011

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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