Skip to main content
padlock icon - secure page this page is secure

Genomic Analysis of Cytotoxicity Response to Nanosilver in Human Dermal Fibroblasts

Buy Article:

$106.67 + tax (Refund Policy)

The aim of this paper was to investigate the molecular mechanisms of interaction between nanosilver and human dermal fibroblasts-fetal (HDF-f) at the level of gene expression. After HDF-f was treated with nanosilver for 1, 4 and 8 h, the cellular response was evaluated with methylthiazoltetrazolium (MTT) assay and flow cytometry analysis. Global gene expression profiles were examined using Illumina Human-6_V3 Expression BeadChip Array and the results were verified by quantitative real-time polymerase chain reaction (RT-PCR). The obtained differential expressed genes were analyzed by the integration of clustering, gene ontology (GO) and biological pathway analysis. The results suggest that nanosilver may cause disruption of cytoskeleton and cellular membrane, disturbance of energy metabolism and gene expression associated pathways, and DNA damage accompanied by cell cycle arrest. When the nanoparticle-cell interaction mechanisms induced by nanogold in our previous research were compared with nanosilver in the present study, both the similarities and differences underlying biological processes and gene regulations were found. The research also suggests that the genomics research can provide a convenient and efficient approach to the understanding of cytotoxicity mechanisms of nanomaterials.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Publication date: April 1, 2011

More about this publication?
  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more