In Vitro and In Vivo Evaluation of Linear Polyethylenimine Nanoparticles
bPEI (polyethylenimine, 25 kDa, gold standard) is highly effective in transfection efficiency owing to its high buffering capacity, however, cytotoxicity limits its use in in vivo applications. we hypothesized that partial conversion of secondary amines in lPEI to tertiary amines,
while preserving the overall number of amines, would result in improved buffering capacity, which may, in turn, improve transfection efficiency of the resulting nanoparticles with cell viability comparable to that of native lPEI. lPEI was crosslinked with BDE to obtain a series of lPEI nanoparticles
(LPN-1 to LPN-8) which were obtained in ∼80–85% yield. These particles were relatively non-toxic in vitro and in vivo. In vivo gene expression studies using LPN-5 in Balb/c mice through i.v. injection showed maximum expression of the reporter gene in the
spleen. These results demonstrate the potential of these particles as efficient transfection reagents.
Keywords: NON-TOXIC; POLYETHYLENIMINE NANOPARTICLES; TRANSFECTION REAGENT
Document Type: Research Article
Publication date: 01 January 2011
- Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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