Time-Resolved Luminescence-Based Assay for Thyroglobulin
We have demonstrated a time-resolved luminescence (TRL) assay for the sensitive and selective detection of thyroglobulin (Tg), a thyroid cancer marker, in homogeneous solution using water-soluble α-D-mannose-conjugated Au nanodots (Man-Au NDs). The Man-Au NDs (1.7 nm in diameter) were prepared from the reaction between Au nanoparticles (2.9 nm in diameter) with 11-mercapto-3,6,9-trioxaundecyl-α-D-mannopyranoside (Man-RSH; 0–10 mM). We have found that the luminescence intensity and lifetimes of Man-Au NDs separately become stronger and longer, respectively, upon increasing the concentration of Man-RSH. The Man-Au NDs possess several interesting optical properties, including long luminescence lifetime (>500 ns) and large Stokes' shift (>150 nm). The TRL assay is based on the competition between Tg and Man-Au NDs for interacting with concanavalin A (Con A). The fluorescence intensities integrated over the region of 50–400 ns increase upon increasing the concentration of Tg over the range 0.1–2.5 nM. The Con A/Man-Au NDs system in a label-free manner allows the detection of Tg at concentrations as low as 90 pM in the presence of BSA at 50 M by TRL. With its advantages of rapid and specificity, this present approach holds great potential for diagnosis of cancers.
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Document Type: Research Article
Publication date: October 1, 2009
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