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Preparation and Agglutination of Immuno-Nanolatex for Malaria Diagnosis

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This is the first systematic study on the adsorption of malaria antigen onto latex particles to elaborate immuno-latex for diagnostic purpose. Immuno-latex was prepared from adsorption of malaria antigen onto polystyrene (PS) or poly(styrene-co-acrylic acid) (St/AA) latex particles and was used for detection of antibody to P. Falciparum based on agglutination test. Adsorption behavior of the malaria antigen onto polymer particles as a function of incubation time, antigen concentration, pH and concentration of the medium was studied. The prepared immuno-latexes were characterized in terms of immuno-agglutination and specific bridging using patient's plasma with P. Falciparum infection. This evaluation was performed using photon correlation spectroscopy.
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Keywords: AGGLUTINATION; IN VITRO TEST; LATEX; MALARIA; PROTEIN ADSORPTION

Document Type: Research Article

Publication date: October 1, 2009

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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