The Accuracy of Amplex Red Assay for Hydrogen Peroxide in the Presence of Nanoparticles
We have shown the ability of different types of oxide nanoparticles, CeO2, Fe2O3, TiO2 and SnO2 to skew the accuracy of detection of H2O2 using the widely applied Amplex Red assay. These oxide materials are commonly employed in electronics manufacture, industrial catalysis, and in the manufacture of consumer products, and their toxicological profiles are not well understood. All four oxide nanoparticles demonstrated intrinsic capability of converting Amplex Red molecules to fluorescent resorufin, even in the absence of H2O2, in aqueous solution as well as cell culture media. Kinetic parameters were calculated and a simulated time dependent concentration profile was generated to better understand this complicated system. The results show that care is needed for interpreting the result of seemly robust bioassays in the presence of nanoparticles.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
Document Type: Research Article
Publication date: October 1, 2009
More about this publication?
- Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Terms & Conditions
- Ingenta Connect is not responsible for the content or availability of external websites