Skip to main content
padlock icon - secure page this page is secure

Aptamer-Functionalized Au Nanoparticles for the Electrochemical Detection of Thrombin

Buy Article:

$106.38 + tax (Refund Policy)

An aptamer-based sandwich assay with electrochemical detection for thrombin analysis is reported using Au nanoparticles. The primary aptamer was immobilized on the surface of a screen-printed carbon electrode (SPCE) and the secondary aptamer was immobilized on Au nanoparticles. The electrochemical reduction current response of Au nanoparticles was monitored for the quantitative detection of thrombin. The limit of detection was determined as 1 nM. The proposed work demonstrates the high specificity of aptamers together with the use of Au nanoparticles. The effect of interfering proteins such as bovine serum albumin (BSA) was investigated. Control experiments also involved the use of an aptamer that has a binding affinity to immunoglobulin E (IgE). Aptamer-based analysis using Au nanoparticles opens the possibility of applications in complex biological fluids and a step closer towards diagnostics or medical investigation.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Publication date: June 1, 2008

More about this publication?
  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more