Increased Osteoblast Functions on (Poly-lactic-co-glycolic acid) with Highly Dispersed Nanophase Titania
Bone injuries, diseases and genetic malformations often require bone substitutes. Current bone substitute materials do not exhibit the physiological or mechanical characteristics of true bones. Much work is needed in the design of more effective bone tissue engineering materials to effectively induce the growth of normal bone tissue. Nanotechnology offers exciting alternatives to traditional bone implants since bone itself is a nanostructured material composed of nanofibered hydroxyapatite well-dispersed in a mostly collagen matrix. For this purpose, the objective of the present in vitro study was to fabricate polymer composites with well-dispersed nanophase ceramics and then determine bone cell functions on such composites. To accomplish this, nanometer grain size titania was dispersed in a biodegradable poly-lactic-co-glycolic acid (PLGA) matrix by various sonication powers from 0 W to 166.25 W. Osteoblast (bone-forming cell) adhesion and subsequent functions (such as total protein synthesis, alkaline phosphatase synthesis, collagen synthesis, and calcium deposition) on nanophase titania/PLGA composites were investigated in vitro. Results demonstrated that the dispersion of titania in PLGA was enhanced by increasing the intensity of sonication and, more importantly, greater osteoblast adhesion correlated with improved nanophase titania dispersion in PLGA. In addition, results correlated better osteoblast long-term functions, such as alkaline phosphatase activity and calcium-containing mineral deposition, with improved nanophase titania dispersion in PLGA. In this manner, the present study demonstrated that PLGA composites with well-dispersed nanophase titania can improve osteoblast functions necessary for increased orthopedic implant efficacy.
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Document Type: Research Article
Publication date: March 1, 2005
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