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Home / Journal of Biobased Materials and Bioenergy, Volume 16, Number 1

Liquid Chromatography-Mass Spectrometry Characterized Hydrolysate Fractions Possess Anticancer Activity In Vitro

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Authors: Feng, Nianjie 1 ; She, Shaowen 1 ; Hu, Chuanqin 2 ; Wu, Qian 3 ; 

Source: Journal of Biobased Materials and Bioenergy, Volume 16, Number 1, February 2022, pp. 117-126(10)

Publisher: American Scientific Publishers

DOI: https://doi.org/10.1166/jbmb.2022.2151

To find new plant-derived antitumor drugs, hydrolysate and fractions of poplar were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for their inhibitory effect against liver cancer HepG-2 cells. Strong cytotoxicity of hydrolysate was revealed at processing temperature of 180 °C or above. The hydrolysate formed at 180 °C was sequentially extracted using organic solvent to obtain five fractions, and only the diethyl ether-soluble fraction (F De) showed specific cytotoxicity. The eluted fraction of acetone/n-hexane (F 1) was further isolated by column chromatography separation from the F De fraction at a yield of 70%, and could severely decrease cell viability. LC-MS chromatography indicated that 5-hydroxymethyl furfural, ρ-hydroxybenzoic acid and syringaldehyde were the main compounds in the F 1 fraction. Furthermore, the results of flow cytometry, Western blotting, and polymerase chain reaction indicated that syringaldehyde could tightly control the growth and metabolism of HepG-2 cells. The findings could move forward the value-added applications of lignocellulosic hydrolysate on traditional medicines.

Keywords: Autohydrolysis; Cytotoxicity; Fractionation; HepG-2 Cell; Poplar

Document Type: Research Article

Affiliations: 1: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratoy of Industrial Microbiology, National “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Research Center of Food Fermentation Engineering and Technology, Hubei University of Technology, Wuhan, 430068, China 2: Key Laboratory of Cleaner Production and Integrated Resource Utilization of China National Light Industry, Beijing Technology and Business University, Beijing, 100048, China 3: Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratoy of Industrial Microbiology, National “111” Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Research Center of Food Fermentation Engineering and Technology, Hubei University of Technology, Wuhan, 430068, China

Publication date: 01 February 2022

More about this publication?
  • The goal of the creation of a biobased economy is challenging to agriculture, forestry, academia, government and industry. The extractable resources of the Earth are finite, regardless of the quibble over when they will be depleted. The economic, political and social demands for biobased chemicals, materials and energy are expected to radically transform the materials industries, particularly the plastics industry as well as the biofuel industry. These changes will be based on the principles of sustainability, eco-efficiency, industrial ecology, and green chemistry and engineering. In keeping with the growth of knowledge in this field, there is a strong need for a forum to share original research related to biobased materials and bioenergy. The Journal of Biobased Materials and Bioenergy (JBMB) has been created as an international peer-reviewed periodical to fulfill the need for communication in these research areas. This journal will encompass related research activities in all fields of science, engineering and the life sciences.
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