A Method to Create Golden Hull Rice Biological Resources Using CRISPR/Cas9 System
Rice chalcone isomerase gene OsCHI is a recessive gene that controls the color of rice hull. In order to obtain the OsCHI mutants in rice, we used U3 promoter to drive the expression of OsCHI sgRNA, and the corresponding CRISPR/Cas9 genome editing vector was constructed. The recombinant plasmid was transferred to the rice cultivar Taipei309 by agrobacterium-mediated transformation. Sequencing of OsCHI locus in T 0 transgenic rice showed that four mutants were obtained, which included two homozygous deletion mutations and two heterozygous deletion mutations. Further analysis for the T 0 mutants showed that almost all mutants exhibited obvious brown pigmentation in the panicle at heading stage and golden pigmentation in the panicle at maturity. The successful OsCHI editing not only provided a series of rice golden hull mutants but also proved that CRISPR/Cas9 is a powerful mean for rice genetic engineering in scientific and agricultural applications, which has theoretical and practical significance for production of golden hull two-line sterile rice.
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Document Type: Research Article
Publication date: 01 February 2018
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- The goal of the creation of a biobased economy is challenging to agriculture, forestry, academia, government and industry. The extractable resources of the Earth are finite, regardless of the quibble over when they will be depleted. The economic, political and social demands for biobased chemicals, materials and energy are expected to radically transform the materials industries, particularly the plastics industry as well as the biofuel industry. These changes will be based on the principles of sustainability, eco-efficiency, industrial ecology, and green chemistry and engineering. In keeping with the growth of knowledge in this field, there is a strong need for a forum to share original research related to biobased materials and bioenergy. The Journal of Biobased Materials and Bioenergy (JBMB) has been created as an international peer-reviewed periodical to fulfill the need for communication in these research areas. This journal will encompass related research activities in all fields of science, engineering and the life sciences.
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