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Surfactin Variants from Bacillus subtilis natto CSUF5 and Their Antifungal Properities Against Aspergillus niger

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An antifungal Bacillus strain isolated from the traditional fermented food natto was identified and named as B. subtilis natto CSUF5. Antifungal metabolites of the strain were extracted from the medium of the inhibition zone on the dual culture plate. 2 V7-surfactin isoforms (C14–V7, C13–Emet 1-V7) and 3 I/L7-surfactin isoforms (C13–I/L7, C13,14–Emet 1–I/L7 were identified from the RP-HPLC isolations by MS n (n = 1,2,3) analysis. Both V7-surfactins and I/L7 -surfactins showed slight antifungal activity against A. niger, and their MIC50 decreases in the order V7 > I/L 7. EDTA was found to be an effective strengthening agent which could cooperate with the surfactin variants and enhance their antifungal activity greatly. Microscopic observations revealed that the surfactin variants could delay the fungal spore germination and inhibit the hyphae growth, mainly displayed in hyphal shriveling and distortion. Surfactin variants and EDTA together could lead the hyphae to severe helical warping and fracture. These results will provide promising references for the development of new antifungal products.

Keywords: ANTIFUNGAL ACTIVITY; BACILLUS SUBTILIS NATTO; EDTA; MASS ANALYSIS; SURFACTIN

Document Type: Research Article

Publication date: 01 June 2017

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  • The goal of the creation of a biobased economy is challenging to agriculture, forestry, academia, government and industry. The extractable resources of the Earth are finite, regardless of the quibble over when they will be depleted. The economic, political and social demands for biobased chemicals, materials and energy are expected to radically transform the materials industries, particularly the plastics industry as well as the biofuel industry. These changes will be based on the principles of sustainability, eco-efficiency, industrial ecology, and green chemistry and engineering. In keeping with the growth of knowledge in this field, there is a strong need for a forum to share original research related to biobased materials and bioenergy. The Journal of Biobased Materials and Bioenergy (JBMB) has been created as an international peer-reviewed periodical to fulfill the need for communication in these research areas. This journal will encompass related research activities in all fields of science, engineering and the life sciences.
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