Potential of Indonesian Plants (Annona muricata, Garcinia latissima, and Garcinia celebica) Against Hepatitis C Virus

Development of antiviral drugs for the treatment of Hepatitis C obtained from medicinal plants is an alternative or additional treatment in the therapy of hepatitis C. In this study, we are focused on screening the anti-HCV activity against JFH1 strain genotype 2a with using Indonesia plants including Annona muricata from leaves, and Garcinia latissima and Garcinia celebica from stem bark. The extractions used maceration method were started from n-hexane solvent and the residue continues used methanol solvent for Annona muricata and Garcinia latissima, while Garcinia celebica used acetone solvent. The extracts were then examined with a single dose 20 μg/mL for anti-HCV activity against JFH1 strain genotype 2a and cytotoxicity against Huh7it-1 cell line. Annona muricata and Garcinia celebica extracts exhibited no infection (0%) of HCV and low infections (5.7%) of Garcinia latissima extract. No apparent cytotoxicity was observed in Huh7it-1 cells treated on Garcinia latissima extract, while on Annona muricata and Garcinia celebica extracts exhibited toxicity was high 81.3% and 99.7% against cell line Huh7it-1. These results suggest that Annona muricata, Garcinia latissima and Garcinia celebica have potential as anti-HCV. Further study, purification of the active compounds is required to know the antiviral compound against HCV.