Performance of Gluconeogenesis in Rat Kidneys Due to Systemic Hypoxia

Systemic hypoxia can increase glucose consumption and lactate production in the tissues or organs. The kidneys are the second organs that can perform gluconeogenesis process beside the liver. This study aims were to determine the glucose and lactate concentration in hypoxia induced rat kidneys. How the performance of gluconeogenesis in systemic hypoxia rat kidney? This experimental study used Sprague-Dawley rats that were divided into 6 groups: control, systemic hypoxia on day 1, 3, 5, 7, and 14. Glucose was measured using Glucose kit reagents Cat no. ST 013-0248 B. Lactate was measured using L-lactate RANDOX: Kit (LAC) PAP enzymatic Determination of L-Lactate Manual Cat No. RX Monza. LC 2389. Data were analyzed using two way ANOVA. The results of this study showed the significant difference of the glucose concentration between normoxia (control) and 5 days and 14 days hypoxia (p < 0.05). Analysis showed significant differences in lactate concentration between normoxia and 1 day, 3 days, 5 days, 7 days, and 14 days hypoxia (p < 0.05). The results showed kidney can adapt to hypoxic conditions by increase of glucose production from lactate in the process of gluconeogenesis. There is a difference between the glucose consumption of rat kidneys normoxia with renal mice exposed to hypoxia. On the day of the hypoxia 5, 7, and 14, kidney tissue does not consume glucose given but produces glucose. Glucose levels after incubation showed significant differences between glucose levels normoxia rat kidneys with hypoxic renal rats on day 5 and 14 hypoxia. The lactate production of normoxia renal rats and hypoxia ones were significantly different. Kidney can adapt to hypoxia due to kidney can perform gluconeogenesis.