Biological Activities of Bacillus sp. from Deep Sea Sediment of Makassar Strait

Antibiotic producer bacteria from deep sea sediment in Makassar Strait had been explored in our previous study. In this study, biological activity from one of the strongest bacteria that identified as Bacillus sp. was studied further. Antioxidant activity of the ethyl acetate extract was determined by DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging method. Examination of the cytotoxicity was done using brine shrimp lethality test (BSLT). The values of MIC were determined by using micro dilution technique. Chemical constituents of the extract were also investigated by using LC-MS. The IC₅₀ exhibited weak antioxidant activity with the value at 502.79 μg/ml. Value of the cytotoxic activity was also weak with EC₅₀ value at 480 μg/ml. The MIC value against Staphylococcus aureus, Bacillus substillis and Vibrio eltor were 7,81 μg/ml, 62,5 μg/ml, and 31,25 μg/ml, respectively. Library analysis of LC-MS spectra from the strongest peak showed that the compound could be bengamide E. Relatively new bioactivities from bacteria of deep sea sediment from Makassar Strait Indonesia were showed in this report. Although it has weak antioxidant and cytotoxic activities, it showed interestingly strong and narrow antibacterial activity against S. aureus. Single strain sample was deposited for further investigation.