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Benguk Soybean Cake Extract as the Stimulant of the Number and Motility of Mus Musculus Spermatozoa

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Background: In the last ten years, the incidence of human infertility in Indonesia has been increasing into 10–15% of the population. Therefore, an affordable natural material such as soybean cake from koro benguk (Mucuna pruriens) seeds which content high L-dopa may help in the infertility prevention of male reproductive system. The aim of this study was to analyze the effect of benguk soybean cake extract exposure on the amount and motility of mus musculus spermatozoa. Method: Benguk soybean cake was made from marinated white koro benguk seeds for two days with the water replacement of 4–5 times/day, boiled < 1 hour, mixed with 10 ml extract lemon/1.5 kilograms to reach pH = 5, and processed at room temperature of 30 °C with 65% relative humidity. Benguk soybean cake was extracted by M-WELL 1299 method. Three groups were set as control and the three other groups were given Benguk soybean cake extract treatment of 35, 70, and 140 mg/kgBW/day respectively for 51 days. Results: The results showed that benguk soybean cake extract of 140 mg/kg/day for 51 days increased the number of mus musculus spermatozoa, compared to the negative control (p = 0, 000) and positive control groups (p = 0.001). Exposure of benguk soybean cake extract of 140 mg/kg/day for 51 days increased the motility of mus musculus spermatozoa, compared to the negative control (p = 0.000) and the positive control groups (p = 0.001). Conclusion: Benguk soybean cake extract of 140 mg/kg/day for 51 days increased the number and motility of mus musculus sprematozoa.
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Keywords: Benguk Soybean Cake Extract; L-Dopa; Motility; Mus Musculus; Spermatozoa

Document Type: Research Article

Affiliations: 1: Department of Biostatistics and Population Study, Public Health, Diponegoro University, Semarang, 50275, Indonesia 2: Diploma Program of Chemical Engineering, Faculty of Engineering, Diponegoro University, Semarang, 50275, Indonesia

Publication date: April 1, 2017

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