Isolation and Identification Bacterial Endophyte of Sweet Potato (Ipomoea batatas L.) and Its Antibacterial Test Against Bacterial Pathogen
Background: Bacterial endophyte potentially have antimicrobial properties. The purpose of this research was to identify the natural antibacterial from bacterial endophyte of different strain of sweet potato (purple, white, yellow and orange) against the growth of bacteria S. pyogenes, S. aureus, and S. thypimurium. This research includes testing of isolation and identification of bacterial endophyte found on sweet potatoes, phytochemicals, and carotene content. Method: Experimental method was used in this study. Data were analyzed using analysis of variance (ANOVA) with confidence level of 95% and Tukey HSD test were applied when significant difference obtained in bacterial isolates of endophytic. Results: Fourteen isolates of endophytic bacteria origin were obtained from the sweet potato of purple, white, yellow and orange. Most of endophytic bacteria have potential of antibacterial activity that originally came from white sweet potatoes. Antibacterial potency test demonstrated that the highest inhibition zone on the growth of S. Typhimurium was obtained from the isolate Rooultella planticola > Enterobacter cloacoe complex > Pseudomonas aeruginosa and Enterobacter asburiae with the diameter average 43.5 > 18 > 13 and 11 mm or 180.64 > 32.26 > 58.06 and 32.26% of the inhibitory zone chloramphenicol 30 μg, respectively. Pseudomonas aeruginosa endophytic bacteria isolates suppressed the growth of S. aureus with an average inhibition zone diameter of 15.5 mm, or 27.9% of the inhibitory zone of gentamicin 30 μg. Conclusion: Bacterial endophyte from the strain purple, white, yellow and orange produced a better antibacterial activity against the bacterial growth of S.thypimurium.
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Document Type: Research Article
Affiliations: Department Biology, Faculty of Mathematic and Natural Science, Padjadjaran University, 45363, Indonesia
Publication date: April 1, 2017
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