Background: School children in agriculture area in Brebes District are involved agriculture activities. Pesticide metabolites were detected in 31.25% out of 48 school children. Pesticide activated de-iodinase type 3 enzymes and produce reverse Triiodothyronine (rT3). Reverse
T3 influences uptake of cellular thyroid hormone. The study aimed to describe the pesticide exposure and level of rT3 in school children. Method: Subjects were 84 school children who lived in agriculture area in Brebes District, Indonesia. The study used cross-sectional design. Pesticide
metabolites in urine were measured by HPLC with Triple Quadrupole Tandem Mass Spectrometry detector. Serum samples were examined by ELISA method for rT3 detection. Data were analyzed using Mann-Whitney tests (α = 0.05). Results: In 51.2% out of 84 subjects, three of six
types of dialkyl phosphate metabolite were detected in urine. The type of pesticide metabolites were diethylthiophospate (35.7%), dimethylthiophospate (28.6%), and dimethyldithiophosphate (8.3%). The mean levels of diethylthiophospate were 0.01±0.019 ppm (0.001–0.1 ppm), of dimethylthiophospate
0.015±0.034 ppm (0.001–0.14 ppm), and of dimethyldithiophosphate 0.042±0.013 ppm (0.026–0.064 ppm). The mean level of rT3 was 323.21±193.78 pg/ml (97.22–864.56 pg/ml). All subjects had rT3 above the normal level (25–75 pg/ml). There was a significance
different between the mean level of rT3 among school children who were exposed and non exposed to pesticide (p < 0.001). Conclusion: Pesticides exposures are thought to increase the activity of D3 and have an impact on increasing the levels of rT3 level in school children
in the agriculture area. All subjects may indicate cellular hypothyroidism and needs to be confirmed by assessed free T3/reverse T3 ratio.
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De-Iodinase Type 3;
Document Type: Research Article
Faculty of Public Health Diponegoro University, Semarang, 50275, Indonesia
Doctoral Program of Medicine and Health, Medical Faculty, Diponegoro University, Semarang, 50275, Indonesia
Publication date: April 1, 2017
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