Annona muricata Associated with Increase Phytohemaglutinin Induced Spleen IL-10 Production of Swiss Mice During Cerebral Malaria Phase
Background: Cerebral malaria (CM) prompts patient death. A. muricata-leaf extract (AME) having anti-plasmodial and immunomodulatory properties may provide advantages for malaria-endemic communities. Anemia of CM-patients is due to reduce interleukine (IL)-10 and increase
macrophage migration inhibitory factor (MIF) production which interfere erythropoiesis. The objective of the study was to determine whether AME influenced spleen-IL-10 and MIF production and Hb or erythrocyte counts during CM-phase. Method: A post test only control group design study
was done by using 30 swiss mice which were randomly divided into 6 groups. The C(+) and C(−) groups were Plasmodium berghei ANKA (PbA)-inoculated and healthy-mice. Healthy-X
1 and X
2 groups received ethanolic-AME 100 and 150 mg/Kg BW/day. PbA-inoculated
3 and X
4 groups received either AME dose. IL-10 and MIF produced by splenocytes stimulated with phytohemaglutinin (PHA) or lipopolysacharide (LPS) ex-vivo, were measured by Elisa. Hb and erythrocyte counts were measured by automatic haematology analyzer.
Results: IL-10 was tested by Kruskal-Wallis (p < 0.0001), then Mann-Whitney showed a significantly higher IL-10 of X
4 than C(+) group (p = 0.003 and p = 0.017). PHA and LPS-induced splenocyte MIF production were tested
by One-way ANOVA and Kruskal-Wallis (p = 0.176 and p = 0.413). Mann-Whitney test showed a lower Hb of C(+), X
3 and X
4 than C(−) groups (p = 0.045, p = 0.038, and p = 0.016). Erythrocyte counts were not different among
6 groups studied (p = 0.072). IL-10 and MIF had no correlation with Hb or erythrocyte counts during CM-phase. The conclusion is that AME associates with the increase spleen-IL-10 production during CM-phase. The spleen-IL-10 and MIF might not influence Hb and erythrocyte counts during
CM-phase. Conclusion: AME associates with the increase spleen-IL-10 production during CM-phase. The spleen-IL-10 and MIF might not influence Hb and erythrocyte counts during CM-phase.
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Document Type: Research Article
Parasitology Department, Medical Faculty, Diponegoro University, Semarang, 50275, Indonesia
Biomedical Science Post Graduate Program, Medical Faculty Diponegoro University, Semarang, 50231, Indonesia
Medical Faculty, Diponegoro University, Semarang, 50275, Indonesia
Biostatistic Department, Public Health Faculty, Diponegoro University, Semarang, 50275, Indonesia
Publication date: April 1, 2017
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