Background: The aim of this research was to determine the preeminent edible mushroom and Minimum Inhibitory Concentration (MIC) of antibacterial activities from the two species of oyster mushrooms namely, Pleurotus ostreatus Jacq and Pleurotus cystidiosus O. K.
Mill, on Escherichia coli Migula. Oyster mushrooms are native to both deciduous hardwood and conifer tree species. Additionally, they are recognized as wood decomposers and ubiquitous in forestlands around the world. Oyster mushrooms in the genus Pleurotus stand out as exceptional
allies for improving human and environmental health. These mushrooms have great reputation, as they are easily reproducible, highly nutritious and medicinally supportive. Method: The research used a descriptive and exploratory method with screening by disc diffusion method with a modified
Kirby-Baueur, and MIC test on the elected fungi extract against Escherichia coli was conducted using the liquid dilution test antibacterial activity with disc diffusion method. Results: The screening test showed that fungi Pleurotus ostreatus had the highest antibacterial
activity indicated with 11.9 mm wide of inhibition zone. On the other hand, Pleurotus cystidiosus had a lower inhibition zone with 11.3 mm wide. The MIC test showed that fungi extract with the concentration of 3.375 mg·mL−1 was able to inhibit the growth of E.
coli Migula. Conclusion: Both fungi extract P. ostreatus and P. cystidiosus were potentially used as antibacterial against E. coli Migula. The MIC of extract was obtained at 3.6 mg·mL−1, while the optimum extract concentration from the
fungi in inhibiting the growth of E. coli was at 3.375 mg·mL−1. This study provided the insight that both oyster mushrooms species are potentially used as bacteriostatic that may overcome the food poisoning problems due to E. coli in Southeast Asian countries.
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Escherichia coli Migula;
Pleurotus cystidiosus O. K. Mill;
Pleurotus ostreatus Jacq
Document Type: Research Article
Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Padjadjaran, Bandung, 40132, Indonesia
Laboratory of Taxonomy, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Padjadjaran, Bandung, 40132, Indonesia
Publication date: April 1, 2017
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