The Use of Non Dairy Creamer Wastewater as the Growth Medium of Saccharomyces cerevisiae for Single-Cell Protein Production

This Non dairy creamer (NDC) wastewater have a potential as microorganism growth medium. This research had an objective to find out the potential of non dairy creamer industrial wastewater as the growing medium for Saccharomyches cerevisiae and protein production within the non dairy creamer wastewater. The research applied a full factorial design, by which treatments composed of multi-factoral combination were used and Multivariate of Anova data analysis. The treatments consisted of P1 (NDC medium concentration 25%), P2 (50%), P3 (75%), and P4 (100%), with the concentration determined for cells of 10⁵, 10⁶, 10⁷, and 10⁸. According to the Multivariate of Anova analysis, the observatory opportunities of the medium and cell concentration resulted in p 0.000, or p < 0.05. Therefore, H₀ was rejected as interaction between the medium concentration with cell concentration and single-cell production existed. The medium concentration determined at 100%, 75%, 50% and 25% resulted in significantly no difference from the number of cells and the cell dry weight at p > 0.05, but significantly difference from the protein content at p < 0.05. The highest protein content was obtained by the medium concentration of 75% and cell concentration of 106 (cell/ml).