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The Interface Nonplanarity Investigated by the Surface Plasmon Resonance Imaging

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Surface plasmon resonance imaging (SPRi) sensors realize highly sensitive, real-time, quantitative, label-free, high-throughput biological interaction monitoring and the binding profiles from multi-analytes. Further provide the binding kinetic parameters between different biomolecules. With such a sensitive method it is very important to point the need for planarity and homogeneity of the entire biochip for a correct output data. This paper is based on experimental SPRi data from our commercial device GenOptics SPRI-Lab+ measured at biochip with Kretschmann configuration. Initial processing of calibration data shows significant variations in the resonance angle across the active layer of the biochip. Here we analyze in detail the possible reasons for observed inhomogeneity, which has a great influence on the operation of the device.
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Keywords: BIOCHIP; ELLIPSOMETRY; SURFACE PLASMONS

Document Type: Research Article

Publication date: March 1, 2016

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  • ADVANCED SCIENCE LETTERS is an international peer-reviewed journal with a very wide-ranging coverage, consolidates research activities in all areas of (1) Physical Sciences, (2) Biological Sciences, (3) Mathematical Sciences, (4) Engineering, (5) Computer and Information Sciences, and (6) Geosciences to publish original short communications, full research papers and timely brief (mini) reviews with authors photo and biography encompassing the basic and applied research and current developments in educational aspects of these scientific areas.
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