Antioxidant Activities of Nanostructured Ginger (Zingiber officinale Roscoe) Rhizome as Affected by Different Milling Time
The aims of this research were to investigate the antioxidant properties of nanostructured ginger as subjected to different milling times. In this study, the ginger rhizome was successfully nanonized using a planetary ball mill process and the effect of milling time has been investigated. The influence of milling at 2, 4, 6, and 8 hours on physical and antioxidant properties of nanostructured ginger were evaluated. The antioxidant properties were identified using a ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays. It was found that the average particle size reduced to 222.3 nm after 4 hours of milling time but agglomerated nanoparticles were observed for sample that continually milled for 6 and 8 hours. The results revealed that higher value of Total Phenolic (356.06 mg GAE/g extract) as well as Total Flavonoid (21.86 mg QE/g extract) in the sample milled for 4 hours whereas the lowest value shown by sample milled for 8 hours. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging reached up to 60% for all tested nanostructured ginger except very low percent of inhibitory shown by sample milled for 6 hours. The IC50 concentration for inhibition of DPPH was proximately at 800 ppm for sample milled for 2 hours, 400 ppm for 4 hours of milling times and 1000 ppm for sample milled for 8 hours. However sample milled for 8 hours showed significantly (p < 0.05) greater capability to reduce ferric among the other tested samples.
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Document Type: Research Article
Publication date: December 1, 2013
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