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Reporting Heavy Metal Resistance Bacterial Strains from Industrially Polluted Sites of Northern India Using Fatty Acid Methyl Ester (FAME) Analysis and Plasma-Atomic Emission Spectroscopy (ICP-AES)

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Microcosm experiments were performed using native sludge, sewage dumps and effluents from different contaminated sites of industry. Using Plasma-Atomic Emission Spectroscopy (ICP-AES) and serial inhibitory test, the microbial composition and activity were characterized in soil contaminated with arsenic (ranges from 1.15–4.62 mg/kg), nickel (ranges from 2.96–38.3 mg/kg), cadmium (ranges from 0–18.72 mg/kg) and chromium (ranges from 7.715–33.28 mg/kg) which were sampled as “Heavy metal resistant bacterias” mentioned as HMR1, HMR2, HMR3 and HMR4 specifically. The growth inhibitory assay results for each of bacterial isolates from the tested heavy metals were performed. The maximum tolerance for HMR1 against arsenic was 500 μg/ml, HMR2 against cadmium was 700 μg/ml, HMR3 against chromium was 250 μg/ml, of HMR4 against nickel was 175 μg/ml. Pure culture was raised from different samples (HMR1 to HMR4) and subjected for Fatty Acid Methyl Esters analysis to confirm them as Bacillus, Stenotrophomonas.
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Document Type: Research Article

Publication date: November 1, 2013

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