Optimization of Extraction Process of Crude Polysaccharides from Fruiting Body of Ganoderma tsugae

Polysaccharide from Ganoderma tsugae has received considerable attention to produce the medicine from nature for health care, treat diabetes and cancer patient, this extracted compound from mushroom can applies to add in food as a biologically active ingredient. Central Composite design (CCD) was employed to optimize the extraction process of crude polysaccharides from fruiting body of G. tsugae. The parameters in this study were the extraction temperature (82–98 °C), the extraction time (199–441 min) and the ratio of water to raw material (13–47 ml/g). The statistical regression analysis showed that a quadratic model was most fitted to estimate the optimal production (R ² = 98.68%). The optimal condition for extraction process was found at 94 °C of extraction temperature, 337 min of extraction time and 27.89 ml/g of ratio of water to raw material. Under these conditions, the predicted value of polysaccharides was 0.719%. These result where then verified under optimal condition.