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Optimization of Extraction Process of Crude Polysaccharides from Fruiting Body of Ganoderma tsugae

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Polysaccharide from Ganoderma tsugae has received considerable attention to produce the medicine from nature for health care, treat diabetes and cancer patient, this extracted compound from mushroom can applies to add in food as a biologically active ingredient. Central Composite design (CCD) was employed to optimize the extraction process of crude polysaccharides from fruiting body of G. tsugae. The parameters in this study were the extraction temperature (82–98 °C), the extraction time (199–441 min) and the ratio of water to raw material (13–47 ml/g). The statistical regression analysis showed that a quadratic model was most fitted to estimate the optimal production (R 2 = 98.68%). The optimal condition for extraction process was found at 94 °C of extraction temperature, 337 min of extraction time and 27.89 ml/g of ratio of water to raw material. Under these conditions, the predicted value of polysaccharides was 0.719%. These result where then verified under optimal condition.
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Document Type: Research Article

Publication date: September 1, 2013

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  • ADVANCED SCIENCE LETTERS is an international peer-reviewed journal with a very wide-ranging coverage, consolidates research activities in all areas of (1) Physical Sciences, (2) Biological Sciences, (3) Mathematical Sciences, (4) Engineering, (5) Computer and Information Sciences, and (6) Geosciences to publish original short communications, full research papers and timely brief (mini) reviews with authors photo and biography encompassing the basic and applied research and current developments in educational aspects of these scientific areas.
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