Designed Pattern Signals of Protein Detection on the Surfaces of Silicon/Silica and Gold/Silica Slides

Bio-microarray is a development tendency of biosensor in order to obtain the most informations of the analytes. For some methods of bio-recognition, silicon, silica and gold matrixes are commonly used as the surfaces of bio-reactions. Two kinds of slides: (i) silica micro-pattern coated silicon matrix; (ii) gold micro-pattern embedded silica matrix were utilized as the surfaces of protein detection. The slide surfaces and fluorescent signals were measured by contact angle, X-ray photoelectron spectroscopy analysis (XPS) and fluorescent microarray scanner. The results suggested the distinct fluorescent patterns were found on the surfaces of both two slides according to the designed patterns, which indicated that the signals on both of the slides didn't interfere with the background. The silicon/silica and gold/silica slides could be used as the support of bio-microarray in the detection methods such as surface plasmon resonance (SPR), imaging ellipsometry, quartz crystal microbalance (QCM) and so on.