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Urea Sensing Properties of Cu-Doped Titanate Nanostructures

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This paper presents the urea sensing properties of Cu-doped titanate nanostructure. Cu-doped nanomaterial was prepared using hydrothermal method at 150 °C for 48 hours followed by calcination at 350 °C. Thick films were deposited using screen printing technique on electropolished aluminum substrates. Morphological changes occurred from particle to mixed structure of particle and flakes during hydrothermal process. Urease was immobilized on the films by soaking in urease solution (100 units) for 5 h which is covalently attached on the surface. In general, conductivity of film increased after urease immobilization as observed in the buffer solution. The conduction linearly increases over the wide range of urea concentration i.e., 1 mM to 500 mM. Urease immobilization was confirmed from IR spectroscopy analysis of the films giving additional peaks.
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Keywords: ENZYME IMMOBILIZATION; THICK FILMS; TITANATE; UREA SENSOR

Document Type: Research Article

Publication date: November 1, 2011

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  • ADVANCED SCIENCE LETTERS is an international peer-reviewed journal with a very wide-ranging coverage, consolidates research activities in all areas of (1) Physical Sciences, (2) Biological Sciences, (3) Mathematical Sciences, (4) Engineering, (5) Computer and Information Sciences, and (6) Geosciences to publish original short communications, full research papers and timely brief (mini) reviews with authors photo and biography encompassing the basic and applied research and current developments in educational aspects of these scientific areas.
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