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Equilibrium Gradient Separation Technology

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The research about biological molecules with an appropriate analysis method is very significant for the development of life science. Equilibrium gradient separation technology is a developing method which is explored for the analysis of biological macromolecules including peptides, protein, nucleic acids (DNA and RNA), and so on. It combines separation and concentration by using an electrophoretic force and an opposing hydrodynamic force to create a stable equilibrium point on which analytes can focus. Therefore, different analytes can be focused on different equilibrium point. Since the establishment of equilibrium gradient separation technology at the end of the 1980s, a family of this technology has been developed. And each one has its own characteristic with the utilization of different theory to form a gradient (in pH, electric field, conductivity, temperature, etc.), which finally induce a gradient in electrophoretic force. This paper briefly introduces the development of equilibrium gradient separation technology and prospects for the future of this technology. And the representative technologies including counteracting chromatographic electrophoresis technology, conductivity gradient focusing technology, temperature gradient focusing technology, electric-field gradient focusing technology, dynamic field gradient focusing technology and bipolar electrode focusing technology are presented.
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Keywords: ANALYTICAL TECHNOLOGY; ELECTRIC-FIELD GRADIENT FOCUSING TECHNOLOGY; EQUILIBRIUM GRADIENT SEPARATION TECHNOLOGY; MICROFLUIDIC CHIP; TEMPERATURE GRADIENT FOCUSING TECHNOLOGY

Document Type: Review Article

Publication date: June 1, 2013

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  • Advanced Chemistry Letters is an international multidisciplinary peer-reviewed journal covering all fundamental and applied research areas of chemical sciences including organic chemistry, inorganic chemistry, synthetic chemistry, medicinal chemistry, analytical chemistry, organometallic chemistry, nuclear chemistry, electrochemistry, atmospheric chemistry, environmental chemistry, materials chemistry, materials science, supramolecular chemistry, physical chemistry, polymer chemistry, bioinorganic chemistry, physical organic chemistry, surface chemistry, biochemistry, molecular biology, chemical biology, food chemistry, natural product chemistry, neurochemistry, pharmacology, photochemistry, photobiology, toxicology, nanoscience, nanotechnology, agrochemistry, green chemistry, marine chemistry, geochemistry, petrochemistry, radiochemistry, astrochemistry, molecular physics, chemical engineering, quantum chemistry, and theoretical and computational chemistry.
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