Simultaneous Determination of Quercetin and Rutin in Pharmaceutical Products by UV-Vis and Multivariate Curve Resolution-Alternating Least Squares
Developing simple, reliable and selective methods for pharmaceutical determination plays an important role to control their quality and safety for clinical applications. In this work, UV-vis spectrophotometry and multivariate curve resolution-alternating least squares (MCR-ALS) were
employed for simultaneous identification and quantitative determination of quercetin and rutin in three medicine samples. The wavelength interval 301–500 nm was selected for MCR-ALS analysis. The recoveries were in the range from 97.81 to 106.38% for quercetin and from 94.87 to 106.95%
for rutin. As a comparison, MCR-ALS was also carried out for the matrix in which the wavelength range was from 200 to 500 nm. It shows that the predictive ability of ALS model is improved by the selected wavelength range and the wavelength selection can also refine the performance of ALS analysis.
The predictive capability of MCR-ALS was also compared to that obtained by partial least squares (PLS) approach. Result shows that the MCR-ALS method has high accuracy for detecting quercetin and rutin in presence of complex unknown interferences in pharmaceutical samples. The method may provide
an alternative technique for analyzing multicomponent samples by using UV-vis spectroscopy.
Keywords: MULTIVARIATE CURVE RESOLUTION-ALTERNATING LEAST SQUARES; QUERCETIN; RUTIN; UV-VIS SPECTROPHOTOMETRY
Document Type: Research Article
Publication date: 01 March 2013
- Advanced Chemistry Letters is an international multidisciplinary peer-reviewed journal covering all fundamental and applied research areas of chemical sciences including organic chemistry, inorganic chemistry, synthetic chemistry, medicinal chemistry, analytical chemistry, organometallic chemistry, nuclear chemistry, electrochemistry, atmospheric chemistry, environmental chemistry, materials chemistry, materials science, supramolecular chemistry, physical chemistry, polymer chemistry, bioinorganic chemistry, physical organic chemistry, surface chemistry, biochemistry, molecular biology, chemical biology, food chemistry, natural product chemistry, neurochemistry, pharmacology, photochemistry, photobiology, toxicology, nanoscience, nanotechnology, agrochemistry, green chemistry, marine chemistry, geochemistry, petrochemistry, radiochemistry, astrochemistry, molecular physics, chemical engineering, quantum chemistry, and theoretical and computational chemistry.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Aims and Scope
- Ingenta Connect is not responsible for the content or availability of external websites
- Access Key
- Free content
- Partial Free content
- New content
- Open access content
- Partial Open access content
- Subscribed content
- Partial Subscribed content
- Free trial content