Immune Function During and After 60 min of Moderate Exercise Wearing Protective Clothing
Jimenez C, Mathieu J, Peinnequin A, Carter R III, Alonso A, Melin B. Immune function during and after 60 min of moderate exercise wearing protective clothing. Aviat Space Environ Med 2008; 79:570–6.
Introduction: As exercise while wearing protective clothing exacerbates body heat storage compared to exercise in the heat, and as exercise alters immune responses, it appeared worthwhile to examine immune and stress responses while wearing protective clothing during moderate exercise. Methods: Eight subjects completed two bouts of exercise at 45% in a thermoneutral environment: once while wearing shorts only (Control trial, CON) and again while wearing protective clothing (PRO). Venous blood samples were taken to analyze TNF-α mRNA by RT-PCR in LPS stimulated blood, plasma catecholamines, and cortisol. Blood cell count was analyzed by flow cytometry. Rectal temperature (Tre) was monitored continuously. Results: Exercise with PRO resulted in significantly greater increases in Tre (39.2 ± 0.2°C in PRO vs. 38.0 ± 0.1°C in CON) and plasma epinephrine and norepinephrine (+70% and 150%, respectively). Plasma cortisol increased only at the end of PRO exercise (+33%). Leukocyte and lymphocyte cell count was 14% and 18% higher, respectively, but there were no significant changes in T cytotoxic and NK cell counts compared to the CON trial. Only T helper lymphocyte count was lower (−29%). During both exercise trials, T helper lymphocytes were significantly decreased at the end of exercise and recovery. With or without protective clothing, exercise was associated with an inhibition of TNF- α expression in stimulated monocytes (∼ −50% at min 20 and 40, and ∼ −30% at min 60). Discussion: Protective clothing wearing induces significant thermal challenge during exercise. The inhibition of TNF-α appears to be mediated primarily by exercise and not the added thermal load associated with protective clothing.
Introduction: As exercise while wearing protective clothing exacerbates body heat storage compared to exercise in the heat, and as exercise alters immune responses, it appeared worthwhile to examine immune and stress responses while wearing protective clothing during moderate exercise. Methods: Eight subjects completed two bouts of exercise at 45% in a thermoneutral environment: once while wearing shorts only (Control trial, CON) and again while wearing protective clothing (PRO). Venous blood samples were taken to analyze TNF-α mRNA by RT-PCR in LPS stimulated blood, plasma catecholamines, and cortisol. Blood cell count was analyzed by flow cytometry. Rectal temperature (Tre) was monitored continuously. Results: Exercise with PRO resulted in significantly greater increases in Tre (39.2 ± 0.2°C in PRO vs. 38.0 ± 0.1°C in CON) and plasma epinephrine and norepinephrine (+70% and 150%, respectively). Plasma cortisol increased only at the end of PRO exercise (+33%). Leukocyte and lymphocyte cell count was 14% and 18% higher, respectively, but there were no significant changes in T cytotoxic and NK cell counts compared to the CON trial. Only T helper lymphocyte count was lower (−29%). During both exercise trials, T helper lymphocytes were significantly decreased at the end of exercise and recovery. With or without protective clothing, exercise was associated with an inhibition of TNF- α expression in stimulated monocytes (∼ −50% at min 20 and 40, and ∼ −30% at min 60). Discussion: Protective clothing wearing induces significant thermal challenge during exercise. The inhibition of TNF-α appears to be mediated primarily by exercise and not the added thermal load associated with protective clothing.
Keywords: TNF- α mRNA; catecholamines; cortisol; exercise; hyperthermia; leukocyte subsets; lymphocyte subsets
Document Type: Research Article
Publication date: 01 June 2008
- The peer-reviewed monthly journal, Aviation, Space, and Environmental Medicine (ASEM) provides contact with physicians, life scientists, bioengineers, and medical specialists working in both basic medical research and in its clinical applications. It is the most used and cited journal in its field. ASEM is distributed to more than 80 nations.
To access volumes 86 to present, please click here. - Information for Authors
- Submit a Paper
- Subscribe to this Title
- Membership Information
- Information for Advertisers
- Submit Articles
- Ingenta Connect is not responsible for the content or availability of external websites
- Access Key
- Free content
- Partial Free content
- New content
- Open access content
- Partial Open access content
- Subscribed content
- Partial Subscribed content
- Free trial content