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Free Content Prolonged Anti-Orthostatic Hind Limb Unloading and Murine Allergic Asthma

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BACKGROUND: Hind limb unloading (HU) is one of the ground-based models of simulated microgravity. As bacterial and viral infections could affect the immune system, the immunologic effect of HU should be studied in a specific-pathogen-free (SPF) laboratory. However, a review of the literature did not reveal any studies on the immunologic effects of prolonged HU in a murine model of allergic disease. Accordingly, the present study was undertaken to evaluate the effect of HU in a murine model of allergic asthma in a SPF laboratory.

METHODS: Twenty BALB/c mice were allocated equally to Group A (control group), Group B (HU group), Group C (allergic group), or Group D (allergic + HU group). Weight gains, serum total and ovalbumin (OVA)-specific IgE, titers of IL-1, IL-5, IL-10, and IFN-γ in bronchoalveolar lavage (BAL) fluid, and histopathologic findings of the lungs were compared.

RESULTS: After 2 wk of HU, Group D showed significantly more weight loss (−2.0 ± 0.2 g) than Group C (−1.1 ± 0.4 g). Groups B and D showed significant increases in serum OVA-specific IgE as compared with Groups A and C. Group D had significantly lower titers of IL-5 (Group C: 53.0 ± 15.2 pg · ml−1, Group D: 21.9 ± 13.9 pg · ml−1), IL-10 (Group C: 430.8 ± 138.3 pg · ml−1, Group D: 217.6 ± 51.2 pg · ml−1), and IFN-γ (Group C: 104.3 ± 37.5 pg · ml−1, Group D: 36.7 ± 12.8 pg · ml−1) in BAL fluid than Group C. Peri-bronchiolar and pulmonary infiltrations of inflammatory cells were significantly greater in Group D than in Group C.

CONCLUSIONS: Prolonged HU may cause significant weight loss and aggravate disease courses.

Jang TY, Heo M-J, Jung A-Y, Kim YH. Prolonged anti-orthostatic hind limb unloading and murine allergic asthma. Aerosp Med Hum Perform. 2015; 86(9):803–807.
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Keywords: allergy and immunology; asthma; hind limb suspension; specific pathogen-free organisms

Document Type: Research Article

Affiliations: Department of Otorhinolaryngology, Head and Neck Surgery, Inha University College of Medicine, Incheon, Republic of Korea

Publication date: September 1, 2015

More about this publication?
  • This journal (formerly Aviation, Space, and Environmental Medicine), representing the members of the Aerospace Medical Association, is published monthly for those interested in aerospace medicine and human performance. It is devoted to serving and supporting all who explore, travel, work, or live in hazardous environments ranging from beneath the sea to the outermost reaches of space. The original scientific articles in this journal provide the latest available information on investigations into such areas as changes in ambient pressure, motion sickness, increased or decreased gravitational forces, thermal stresses, vision, fatigue, circadian rhythms, psychological stress, artificial environments, predictors of success, health maintenance, human factors engineering, clinical care, and others. This journal also publishes notes on scientific news and technical items of interest to the general reader, and provides teaching material and reviews for health care professionals.

    To access volumes 74 through 85, please click here.
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