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Single-Laboratory Validation of a Method for the Determination of Phenols and Phenates in Disinfectant Formulations by Liquid Chromatography with UV Detection

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A single-laboratory validation study was conducted for an LC method using UV detection for the simultaneous determination of the active ingredients o-phenylphenol (OPP), p-tert-amylphenol (PTAP), and o-benzyl-p-chlorophenol (OBPCP) in disinfectant formulations. Samples were extracted, the extracts diluted with acidified methanol, and the active ingredients separated by LC with a gradient mobile phase and quantified by using UV detection at 285 nm. For each active ingredient, the RSD was 3.7, and the intermediate reproducibility was 3.4. The active ingredient content of the spiked samples analyzed in this study ranged from 0.075 to 10.1 for the individual phenol active ingredients. The average recovery ranges were 86.7104.9, 82.8115.6, and 91.6114.7 for the active ingredients OPP, PTAP, and OBPCP, respectively, for the concentration range of 0.07510.1. This method, with a relatively short chromatographic run time (about 15 min), proved to be reliable and convenient for analyses of products or samples containing all or a combination of these phenol active ingredients.
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Document Type: Research Article

Affiliations: 1: Maryland Department of Agriculture, State Chemist Section, 50 Harry S. Truman Pkwy, Annapolis, MD 21401. 2: U.S. Environmental Protection Agency, Office of Pesticide Programs, Analytical Chemistry Branch, 701 Mapes Rd, Ft. George G. Meade, MD 20755.

Publication date: May 1, 2010

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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