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Simultaneous Determination of Imipramine Hydrochloride and Chlordiazepoxide in Pharmaceutical Preparations by Spectrophotometric, RP-HPLC, and HPTLC Methods

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A binary mixture of imipramine HCl and chlordiazepoxide was determined by three different methods. The first involved determination of imipramine HCl and chlordiazepoxide using the first derivative spectrophotometric technique at 219 and 231.5 nm over the concentration ranges of 120 and 224 g/mL with mean accuracies of 99.47 0.78 and 101.43 1.20, respectively. The second method utilized RP-HPLC with methanolacetonitrile0.065 M ammonium acetate buffer (45 + 25 + 30, v/v/v, pH adjusted to 5.6 0.02 with phosphoric acid) as the mobile phase pumped at a flow rate of 1.0 mL/min. Quantification was achieved using UV detection at 240 nm over concentration ranges of 0.254.0 and 0.11.6 g/mL, with mean accuracies of 101.17 0.56 and 100.67 0.40 for imipramine HCl and chlordiazepoxide, respectively. The third method was HPTLC with carbon tetrachlorideacetonetriethylamine (pH 8.3; 6 + 3 + 0.3, v/v/v) as the mobile phase. Quantification was achieved with UV detection at 240 nm over concentration ranges of 50600 and 20240 ng/spot with mean accuracies of 99.51 0.59 and 100.59 0.84 for imipramine HCl and chlordiazepoxide, respectively. The suggested procedures were checked using prepared mixtures, and were successfully applied for the analysis of pharmaceutical preparations. The accuracy and precision of the methods were confirmed when the standard addition technique was applied. The results obtained by applying the proposed methods were statistically analyzed.
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Document Type: Research Article

Affiliations: Shree S.K. Patel College of Pharmaceutical Education and Research, Department of Pharmaceutical Chemistry, Ganpat Vidyanagar, Kherva, Mehsana-382711, Gujarat, India.

Publication date: May 1, 2010

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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