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Rapid HPLC Determination of Pioglitazone in Human Plasma by Protein Precipitation and Its Application to Pharmacokinetic Studies

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A simple and rapid HPLC method with UV detection was developed for the determination of pioglitazone in human plasma. The method was based on protein precipitation using perchloric acid on an ODS column. The mobile phase consisted of a mixture of phosphate buffer, methanol, acetonitrile, and 12 M perchloric acid (54 + 33 + 12 + 1, v/v/v/v). The UV detector was set at 269 nm. Under these conditions, the retention time of pioglitazone was 5.2 min. The standard curve was linear over the range of 502000 ng/mL pioglitazone in human plasma. The within-day and between-day precision studies showed high reproducibility, with CV less than 5. The LOQ was 44.2 ng/mL. The method has been applied to a bioequivalence study after administration of pioglitazone as 30 mg tablets to 12 healthy volunteers.
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Document Type: Research Article

Affiliations: 1: Tabriz University of Medical Sciences, Department of Pharmaceutics, Faculty of Pharmacy, Tabriz, Iran, and Zanjan University of Medical Sciences, Department of Pharmaceutics, School of Pharmacy, Zanjan, Iran. 2: Tabriz University of Medical Sciences, Department of Pharmaceutics, Faculty of Pharmacy, Tabriz, Iran, and Tabriz University of Medical Sciences, Research Center for Pharmaceutical Nanotechnology, Tabriz, Iran. 3: Tabriz University of Medical Sciences, Department of Pharmaceutics, Faculty of Pharmacy, Tabriz, Iran, and Tabriz University of Medical Sciences, Drug Applied Research Center, Tabriz, Iran.

Publication date: May 1, 2010

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  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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