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Determination of Bioactive Compounds in Cortex Phellodendri by High-Performance Liquid Chromatography

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An HPLC method combined with a photodiode array detector was developed for quantitative determination of five bioactive compounds that belong to two subclasses, including limonin, phellodendrine, jatrorrhizine, palmatine, and berberine in Cortex Phellodendri. The analysis was performed on an Agilent Diamonsil C18 column (4.6 250 mm, 5 m) using a gradient of acetonitrile and 0.3 aqueous diethylamine phosphate (v/v), a flow rate of 0.8 mL/min, and a detection wavelength of 220 nm. The calibration curve was linear over the range of 2.5100.0 g/mL for both phellodendrine and jatrorrhizine, 5.0200.0 g/mL for palmatine, and 7.5300.0 g/mL for both berberine and limonin. The average recoveries ranged from 97.56 to 102.53 with RSD 1.00. Samples from different geographical locations were analyzed to evaluate the applicability of the established method, and the results indicated that the method was efficient, sensitive, and reliable for determining limonin and four alkaloids in Cortex Phellodendri.
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Document Type: Research Article

Affiliations: 1: Changzheng Hospital, Second Military Medical University, Department of Pharmacy, 415 Fengyang Rd, Shanghai 200003, People's Republic of China. 2: Changzheng Hospital, Second Military Medical University, Department of Pharmacy, 415 Fengyang Rd, Shanghai 200003, China and Second Military Medical University, School of Pharmacy, 325 Guohe Rd, Shanghai 200433, People's Republic of China. 3: Changzheng Hospital, Second Military Medical University, Department of Pharmacy, Shanghai 200003, People's Republic of China. 4: Second Military Medical University, School of Pharmacy, 325 Guohe Rd, Shanghai 200433, People's Republic of China.

Publication date: May 1, 2010

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  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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