Chiral Separation of Diastereoisomers of Cefuroxime Axetil by High-Performance Thin-Layer Chromatography
Conditions for separation and determination of diastereoisomers of cefuroxime axetil by HPTLC on cellulose layers with densitometry were developed. A good separation of the constituents was achieved using a mobile phase composed of 1 aqueous -cyclodextrinmethanol (15 + 1, v/v). For detecting spots on the chromatograms, densitometric measurements were used at 285 nm. The method is characterized by high sensitivity; the LOD was 0.04 g/spot and LOQ was 0.11 g/spot for both forms of the cefuroxime axetil diastereoisomers. For the individual diastereoisomers, high recovery values from 96.63 to 104.16 were obtained. Identification of the diastereoisomers was performed by 1H-NMR spectrometric analysis.
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Document Type: Research Article
Affiliations: Jagiellonian University, Collegium Medicum, Department of Inorganic and Analytical Chemistry, Medyczna 9, 30-688 Krakw, Poland.
Publication date: May 1, 2010
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