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Determination of Linezolid in Pharmaceutical Dosage Forms by Liquid Chromatography and Ultraviolet Spectroscopy

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A liquid chromatographic (LC) method and a UV spectrophotometric method were developed and validated for quantitative determination of linezolid in tablets and injection. The isocratic LC analyses were performed on an RP18 column using a mobile phase composed of methanolwateracetonitrile (40 + 40 + 20, v/v/v) at a flow rate of 1.0 mL/min. The UV spectro- photometric method was performed at 251 nm. The analytical methods were validated according to International Conference on Harmonization guidelines. The calibration graphs were linear (correlation coefficient >0.999) in the studied concentration ranges of 0.1 to 10 g/mL for LC and 2 to 16 g/mL for UV spectrophotometry. The relative standard deviation values for intraday and interday precision studies were <2, and the accuracy was >98 for both methods. The specificity of the LC method was proved using forced degradation. Statistical analysis showed no significant difference between the results obtained by the 2 methods. The proposed methods are precise and accurate and can be applied directly to the oral and parenteral pharmaceutical preparations of linezolid.
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Document Type: Research Article

Affiliations: Shree S.K. Patel College of Pharmaceutical Education and Research, Ganpat Vidyanagar, Kherava-382 711, Mehsana, Gujarat, India.

Publication date: September 1, 2007

More about this publication?
  • The Journal of AOAC INTERNATIONAL publishes refereed papers and reviews in the fields of chemical, biological and toxicological analytical chemistry for the purpose of showcasing the most precise, accurate and sensitive methods for analysis of foods, food additives, supplements and contaminants, cosmetics, drugs, toxins, hazardous substances, pesticides, feeds, fertilizers and the environment available at that point in time. The scope of the Journal includes unpublished original research describing new analytical methods, techniques and applications; improved approaches to sampling, both in the field and the laboratory; better methods of preparing samples for analysis; collaborative studies substantiating the performance of a given method; statistical techniques for evaluating data. The Journal will also publish other articles of general interest to its audience, e.g., technical communications; cautionary notes; comments on techniques, apparatus, and reagents.
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